Title

Image processing method and system for analyzing a multi-channel image obtained from a biological tissue sample being stained by multiple stains

Inventors

Michael Barnes
Carlo Bifulco, Earle A. Chiles Research Institute, Robert W. Franz Cancer Research Center, Providence Portland Medical Center, Portland, ORFollow
Ting Chen
Alisa Tubbs

Document Type

Patent

File Date

11-30-2016

Keywords

genomics

Abstract

Systems and methods for automatic FOV selection in immunoscore computation that involve reading images for individual markers from an unmixed multiplex slide or single stain slides, and computing the tissue region mask from the individual marker image. The heat map of each marker is determined by applying the low pass filter on the individual marker image channel and selecting the top K highest intensity regions from the heat map as the candidate FOVs for each marker. The candidate FOVs from the individual marker images are merged together in the same coordinate system by either adding all of the FOVs together or by only adding the FOVs from the selected marker images depending on the user's choice, and registering all the individual marker images to a common coordinate system and transferring the FOVs back to the original images.

Department

Earle A. Chiles Research Institute

Location

United States

Comments

Patent Number: 10,275,880

Assignee

Ventana Medical Systems, Inc. (Tucson, AZ); Providence Health & Services--Oregon (Portland, OR)

Family ID

53276870

Serial Number

15/365,831

Tech ID

14-03-INV

Recommended Citation

Barnes, Michael; Bifulco, Carlo; Chen, Ting; and Tubbs, Alisa, "Image processing method and system for analyzing a multi-channel image obtained from a biological tissue sample being stained by multiple stains" (2016). Technology Transfer - Patents. 27.
https://digitalcommons.providence.org/technologytransfer/27