Title

Isolation of Breast cancer CTCs with multitargeted buoyant immunomicrobubbles.

Document Type

Article

Publication Date

1-1-2018

Keywords

Antibody; Breast cancer; CTC; EGFR; EpCAM; Microbubbles; Biomarkers, Tumor/metabolism; Breast Neoplasms/blood; Breast Neoplasms/pathology; Cell Line, Tumor Cell Separation/methods; Epithelial Cell Adhesion Molecule/metabolism; Female; Humans; Keratins/metabolism; MCF-7 Cells; Microbubbles; Neoplastic Cells, Circulating/metabolism; Neoplastic Cells, Circulating/pathology

Abstract

Circulating tumor cells (CTCs) are extremely rare cells found in blood of metastatic cancer patients. There is a need for inexpensive technologies for fast enrichment of CTCs from large blood volumes. Previous data showed that antibody-conjugated lipid shell immuno-microbubbles (MBs) bind and isolate cells from biological fluids by flotation. Here, blood-stable MBs targeted to several surface markers for isolation of breast tumor cells were developed. MBs coated with anti-human EpCAM antibodies showed efficient binding of EpCAM

Clinical Institute

Cancer

Department

Oncology

Volume

161

First Page

200

Last Page

209

PubMed ID

29080504

Journal Title

Colloids and surfaces. B, Biointerfaces

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