Engineered SH2 Domains for Targeted Phosphoproteomics.
ACS Chem Biol
washington; isb; Humans; Mass Spectrometry; Phosphotyrosine; Protein Binding; Proteome; src Homology Domains
A comprehensive analysis of the phosphoproteome is essential for understanding molecular mechanisms of human diseases. However, current tools used to enrich phosphotyrosine (pTyr) are limited in their applicability and scope. Here, we engineered new superbinder Src-Homology 2 (SH2) domains that enrich diverse sets of pTyr-peptides. We used phage display to select a Fes-SH2 domain variant (superFes; sFes
Institute for Systems Biology
Martyn, Gregory D; Veggiani, Gianluca; Kusebauch, Ulrike; Morrone, Seamus R; Yates, Bradley P; Singer, Alex U; Tong, Jiefei; Manczyk, Noah; Gish, Gerald; Sun, Zhi; Kurinov, Igor; Sicheri, Frank; Moran, Michael F; Moritz, Robert L; and Sidhu, Sachdev S, "Engineered SH2 Domains for Targeted Phosphoproteomics." (2022). Articles, Abstracts, and Reports. 6360.