Document Type
Article
Publication Date
12-18-2020
Publication Title
STAR Protoc
Keywords
washington; seattle; ISB
Abstract
Quantitative changes in transcription factor (TF) abundance regulate dynamic cellular processes, including cell fate decisions. Protein copy number provides information about the relative stoichiometry of TFs that can be used to determine how quantitative changes in TF abundance influence gene regulatory networks. In this protocol, we describe a targeted selected reaction monitoring (SRM)-based mass-spectrometry method to systematically measure the absolute protein concentration of nuclear TFs as human hematopoietic stem and progenitor cells differentiate along the erythropoietic lineage. For complete details on the use and execution of this protocol, please refer to Gillespie et al. (2020).
Department
Institute for Systems Biology
Recommended Citation
Gillespie, Mark A; Palii, Carmen G; Sanchez-Taltavull, Daniel; Perkins, Theodore J; Brand, Marjorie; and Ranish, Jeffrey A, "Absolute quantification of transcription factors in human erythropoiesis using selected reaction monitoring mass spectrometry." (2020). Articles, Abstracts, and Reports. 4342.
https://digitalcommons.psjhealth.org/publications/4342
