The Importance of Standardization on Analyzing Circulating RNA.

Document Type

Article

Publication Date

6-1-2017

Publication Title

Mol Diagn Ther

Keywords

Biomarkers, Tumor; Blood Platelets; Cell-Free Nucleic Acids; Diet; Exercise; Female; Genetic Markers; High-Throughput Nucleotide Sequencing; Humans; Male; MicroRNAs; Polymerase Chain Reaction; Specimen Handling; Time Factors

Abstract

Circulating RNAs, especially microRNAs (miRNAs), have recently emerged as non-invasive disease biomarkers. Despite enthusiasm and numerous reports on disease-associated circulating miRNAs, currently there is no circulating miRNA-based diagnostic in use. In addition, there are many contradictory reports on the concentration changes of specific miRNA in circulation. Here we review the impact of various technical and non-technical factors related to circulating miRNA measurement and elucidate the importance of having a general guideline for sample preparation and concentration measurement in studying circulating RNA.

Clinical Institute

Cancer

Department

Institute for Systems Biology

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