Blotting, Western; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p27; Female; Glycogen Synthase Kinase 3 beta; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Male; Melanoma; NADPH Oxidase 5; Ovarian Neoplasms; Phosphorylation; Prostatic Neoplasms; Proto-Oncogene Proteins c-akt; RNA Interference; Reactive Oxygen Species; Signal Transduction
NADPH oxidase 5 (NOX5) generated reactive oxygen species (ROS) have been implicated in signaling cascades that regulate cancer cell proliferation. To evaluate and validate NOX5 expression in human tumors, we screened a broad range of tissue microarrays (TMAs), and report substantial overexpression of NOX5 in malignant melanoma and cancers of the prostate, breast, and ovary. In human UACC-257 melanoma cells that possesses high levels of functional endogenous NOX5, overexpression of NOX5 resulted in enhanced cell growth, increased numbers of BrdU positive cells, and increased γ-H2AX levels. Additionally, NOX5-overexpressing (stable and inducible) UACC-257 cells demonstrated increased normoxic HIF-1α expression and decreased p27
Antony, Smitha; Jiang, Guojian; Wu, Yongzhong; Meitzler, Jennifer L; Makhlouf, Hala R; Haines, Diana C; Butcher, Donna; Hoon, Dave S; Ji, Jiuping; Zhang, Yiping; Juhasz, Agnes; Lu, Jiamo; Liu, Han; Dahan, Iris; Konate, Mariam; Roy, Krishnendu K; and Doroshow, James H, "NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27" (2017). Articles, Abstracts, and Reports. 2314.