Adenosine Triphosphatases; Cell Nucleus; Chromatin Assembly and Disassembly; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Fungal Proteins; Gene Expression; Nucleosomes; Protein Subunits; Transcription Factors; Yeasts
The SWI/SNF chromatin remodeling complex is highly conserved from yeast to human, and aberrant SWI/SNF complexes contribute to human disease. The Snf5/SMARCB1/INI1 subunit of SWI/SNF is a tumor suppressor frequently lost in pediatric rhabdoid cancers. We examined the effects of Snf5 loss on the composition, nucleosome binding, recruitment, and remodeling activities of yeast SWI/SNF. The Snf5 subunit is shown by crosslinking-mass spectrometry (CX-MS) and subunit deletion analysis to interact with the ATPase domain of Snf2 and to form a submodule consisting of Snf5, Swp82, and Taf14. Snf5 promotes binding of the Snf2 ATPase domain to nucleosomal DNA and enhances the catalytic and nucleosome remodeling activities of SWI/SNF. Snf5 is also required for SWI/SNF recruitment by acidic transcription factors. RNA-seq analysis suggests that both the recruitment and remodeling functions of Snf5 are required in vivo for SWI/SNF regulation of gene expression. Thus, loss of SNF5 alters the structure and function of SWI/SNF.
Institute for Systems Biology
Sen, Payel; Luo, Jie; Hada, Arjan; Hailu, Solomon G; Dechassa, Mekonnen Lemma; Persinger, Jim; Brahma, Sandipan; Paul, Somnath; Ranish, Jeff; and Bartholomew, Blaine, "Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex." (2017). Articles, Abstracts, and Reports. 2298.