Constraint-based modelling captures the metabolic versatility of Desulfovibrio vulgaris.
A refined Desulfovibrio vulgaris Hildenborough flux balance analysis (FBA) model (iJF744) was developed, incorporating 1016 reactions that include 744 genes and 951 metabolites. A draft model was first developed through automatic model reconstruction using the ModelSeed Server and then curated based on existing literature. The curated model was further refined by incorporating three recently proposed redox reactions involving the Hdr-Flx and Qmo complexes and a lactate dehydrogenase (LdhAB, DVU 3027-3028) indicated by mutation and transcript analyses to serve electron transfer reactions central to syntrophic and respiratory growth. Eight different variations of this model were evaluated by comparing model predictions to experimental data determined for four different growth conditions - three for sulfate respiration (with lactate, pyruvate or H
Institute for Systems Biology
Environ Microbiol Rep
Flowers, Jason J; Richards, Matthew A; Baliga, Nitin; Meyer, Birte; and Stahl, David A, "Constraint-based modelling captures the metabolic versatility of Desulfovibrio vulgaris." (2018). All Publications. 131.