Document Type
Article
Publication Date
1-1-2017
Publication Title
Adv Genomics Genet
Keywords
bioinformatics; copy number changes; genomics; viral transformation; whole-genome sequencing
Abstract
Lymphoblastoid cell lines (LCLs) represent a convenient research tool for expanding the amount of biologic material available from an individual. LCLs are commonly used as reference materials, most notably from the Genome in a Bottle Consortium. However, the question remains how faithfully LCL-derived genome assemblies represent the germline genome of the donor individual as compared to the genome assemblies derived from peripheral blood mononuclear cells. We present an in-depth comparison of a large collection of LCL- and peripheral blood mononuclear cell-derived genomes in terms of distributions of coverage and copy number alterations. We found significant differences in the depth of coverage and copy number calls, which may be driven by differential replication timing. Importantly, these copy number changes preferentially affect regions closer to genes and with higher GC content. This suggests that genomic studies based on LCLs may display locus-specific biases, and that conclusions based on analysis of depth of coverage and copy number variation may require further scrutiny.
Department
Institute for Systems Biology
Recommended Citation
Joesch-Cohen, Lena M and Glusman, Gustavo, "Differences between the genomes of lymphoblastoid cell lines and blood-derived samples." (2017). Articles, Abstracts, and Reports. 1212.
https://digitalcommons.providence.org/publications/1212
